biogem: Agilent Bioanalyzer Service

Agilent Bioanalyzer Service

UNIVERSITY OF CALIFORNIA, SAN DIEGO
BIOMEDICAL GENOMICS MICROARRAY CORE FACILITY

RNA Sample Requirements for Agilent Bioanalyzer Analysis

BIOGEM provides a service whereby we run customer RNA samples on the Agilent Bioanalyzer to determine whether the integrity of the RNA sample(s) in question is good. Priority is given to users for whom we are running microarray experiments at a cost of $22 per sample. For investigators not requesting microarray experiments, there is a minimum charge of $220. (Nano full chip – 11 samples/ Pico full chip – 10 samples)

Please note that the turnaround time for Bioanalyzer services not related to microarray experiments is one to two weeks. This is dependent on the number of microarray experiments ongoing.

This form must be filled out completely before we can accept any samples. Once this form has been filled out, please coordinate with James Sprague to drop off samples.

Provide the RNA for Agilent analysis (nanochip) at a concentration range of 0.2 mg to 1 mg per ml or a concentration range of 0.005mg to 0.01 mg per ml (pico chip for samples with limited amount) At least 3 ul is preferred. Label these tubes with with the following information:

Label the labeling aliquot tubes with these information:

An "A" (denoting they are for Agilent analysis).
Your name.
Date of sample submission (day/month/year).
Name of service requested ("Bioanalyzer").

Summary of RNA Requirements:

Amount of RNA REQUIRED
- for nanokit: 3 ul of RNA at a conc. of 0.2 mg to 1 mg per ml.
- for picokit (very small amounts, e.g. laser capture): at least 3 ul.

If the following can information be provided, please do so. If this is not practical i.e. if you are working with very small RNA amounts this information is not necessary.

RNA 260/280 between 1.8-2.0.
Gel validation (if RNA quantities permit).
Ratio of high/low molecular weight rRNA: ~2.

RNA quality will be checked at the facility. If low quality is suspected or the RNA fails to meet our criteria, the experiment will be stopped. Please contact James Sprague if you have any questions or concerns about the integrity of your RNA samples.

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Last Updated March 2009
By Dr. Gary Hardiman

Agilent Bioanalyzer Service

UNIVERSITY OF CALIFORNIA, SAN DIEGO BIOMEDICAL GENOMICS MICROARRAY CORE FACILITY

RNA Sample Requirements for Agilent Bioanalyzer Analysis

Summary of RNA Requirements:

UNIVERSITY OF CALIFORNIA, SAN DIEGO
BIOMEDICAL GENOMICS MICROARRAY CORE FACILITY